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  • Title: [Storage of human bone marrow before transplantation at 4 degrees C. The effect of cell proliferation potential particularly in hematopoietic cloned cell lines].
    Author: Ratajczak MZ, Skorski T, Kuczyński WI, Ratajczak J.
    Journal: Pol Merkur Lekarski; 1998 Mar; 4(21):143-6. PubMed ID: 9640066.
    Abstract:
    The possibility of storage of human bone marrow CD34+ cells at 4 degrees C for bone marrow transplantation purposes was investigated. The cells were placed in Iscove medium supplemented with 20% serum (15% bovine calf serum + 5% human AB serum) for three weeks at 4 degrees C. During storage time clonogenicity of granulocytic-monocytic (CFU-GM) erythropoietic (BFU-E) and megakaryocytic (CFU-Meg) progenitors were investigated. It turns out that it is possible to keep human CD34+ cells at 4 degrees C for at least few days before transplantation. At day four of storage the number of CFU-GM and BFU-E cells still exceeded 50% of cells present at day 0. We have found however, CFU-Meg progenitors in comparison to others are much more sensitive to metabolic storage stress. This enhanced sensitivity of megakaryocytic progenitors could explain at least partially the well known ++phenomenon of retardation of thrombopoietic recovery in patients undergoing bone marrow transplantation.
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