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  • Title: [In vitro study of hemopoietic progenitors in human umbilical cord blood treated with different cytokines and autologous serum].
    Author: Alemán M, Bianchi de Di Risio CC.
    Journal: Medicina (B Aires); 1997; 57(3):299-306. PubMed ID: 9640763.
    Abstract:
    UNLABELLED: The aim was to obtain the ex vivo expansion of human umbilical cord blood (HUCB) cells. A total of 19 samples were assayed to evaluate the number and type of hemopoietic progenitor cells, their proliferating capacity and the stimulating potency of cord blood serum. METHODS: a) CFU-GM, CFU-GEMM and BFU-E cultures in the presence of CSF, BM serum or HUCB serum; b) 35 day LTC in liquid media whether in presence of IL-3 + GM-CSF + SCF or autologous serum (AS). Cells were demidepopulated at 7-day intervals and fresh medium and cytokines were added. Harvested cells were cultured in bone marrow (BM)/ SM and colonies were evaluated after 10 days. RESULTS: The mean number of CFU-GM was similar to BM values; the maximum number of colonies was observed at day 7 and remained high until day 21 whether in addition of cytokines or AS. A total of 8 samples gave rise to colonies up to day 35; these samples showed higher values than BM in SM; HUCB serum has a great stimulating effect on BM cells and HUCB cells compared with nonspecific stimulating factors; moreover, HUCB showed a large dispersion. CONCLUSION: 1) HUCB contains a high number of hemopoietic progenitor cells with a large dispersion coefficient, 2) HUCB plasma has a great stimulatory capacity, 3) it is possible to induce the expansion of HUCB progenitors in LTC either in the presence of cytokines or of AS without loss of potency.
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