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  • Title: Determination of ascorbic acid in nanolitre samples by means of capillary batch injection analysis.
    Author: Backofen U, Matysik FM, Hoffmann W, Ache HJ.
    Journal: Biomed Chromatogr; 1998; 12(3):145-6. PubMed ID: 9646917.
    Abstract:
    Capillary batch injection analysis (CBIA) is applied to the determination of ascorbic acid in nanolitre samples at neutral pH. It is demonstrated that pulsed amperometric detection is superior to constant-potential amperometric detection with respect to signal stability. The calibration plot is linear within the studied concentration range between 0.5 mM and 5 mM and the reproducibility is characterized by a relative standard deviation of 0.80% for 10 successive injections of 188 nL of 3.5 mM ascorbic acid. The attainable sample throughput is up to 500 per hour.
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