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  • Title: Role of ultraviolet A-induced oxidative DNA damage in apoptosis via loss of mitochondrial membrane potential and caspase-3 activation.
    Author: Tada-Oikawa S, Oikawa S, Kawanishi S.
    Journal: Biochem Biophys Res Commun; 1998 Jun 29; 247(3):693-6. PubMed ID: 9647755.
    Abstract:
    UVA has been implicated in multistage photocarcinogenesis. HPLC analysis revealed that the 7,8-dihydro-8-oxo-2'-deoxyguanosine content of DNA in HL-60 cells increased immediately after UVA irradiation. Pulsed field gel electrophoresis showed that cellular DNA strand breakage was induced at 1 h after UVA irradiation. Flow cytometry showed that mitochondrial membrane potential (delta psi m) decreased time-dependently in cells exposed to UVA. The activity of caspase-3 and DNA ladder formation increased at 2 h after UVA irradiation. These results suggest that UVA irradiation induces oxidative DNA damage, followed by the loss of delta psi m and subsequent activation of caspase-3, resulting in apoptosis.
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