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  • Title: A simple technique to isolate DNA and supernatant of genital Mycoplasma hominis and Ureaplasma urealyticum.
    Author: Walther T, Retzlaff C, Stepan H, Faber R.
    Journal: J Perinat Med; 1998; 26(2):123-4. PubMed ID: 9650134.
    Abstract:
    Vaginal colonization with Mycoplasma hominis and Ureaplasma urealyticum has been implicated as a cause of prematurity. Several mechanisms to induce preterm labor have been discussed. The investigation of expressed and secreted enzymes requires a feasible method for culturing and further processing of these bacteria. We describe a simple technique for culturing of Mycoplasma hominis and Ureaplasma urealyticum without contamination with other microorganisms and isolating DNA and supernatant. PCR amplification of a chromosomal Mycoplasma fragment was performed as positive control.
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