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  • Title: Substrate inhibition of cruzipain is not affected by the C-terminal domain.
    Author: Stoka V, McKerrow JH, Cazzulo JJ, Turk V.
    Journal: FEBS Lett; 1998 Jun 12; 429(2):129-33. PubMed ID: 9650575.
    Abstract:
    Endogenous and recombinant cruzipain, the major cysteine proteinase from the protozoan parasite Trypanosoma cruzi, exhibit differences in the protein and circular dichroism spectra probably attributed to the absence of the C-terminal domain in the recombinant enzyme. Substrate hydrolysis of both molecules at 25 degrees C and neutral pH obeyed Michaelis-Menten kinetics whereas significant substrate inhibition was observed above neutral pH. The results suggest that substrate inhibition of cruzipain is pH-dependent, and that the C-terminal domain does not play an essential role in this process.
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