These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: An improved technique for the gas-liquid chromatographic separation of the N-trifluoroacetyl n-butyl derivatives of amino acids.
    Author: Appelqvist LA, Nair BM.
    Journal: J Chromatogr; 1976 Sep 15; 124(2):239-45. PubMed ID: 965460.
    Abstract:
    A complete separation of the twenty protein amino acids and two internal standards in 35 min has been made possible by analyzing their N-trifluoroacetyl n-butyl (TAB) derivatives simultaneously on an ethylene glycol adipate (EGA) column and on an OV-17 column using the same temperature programme. The detector signal from the EGA column is recorded up to the point just after the elution of TAB-aspartic acid; thereafter, the signal from the effluent of the OV-17 column is recorded. The TAB derivative of norleucine is poorly resolved from TAB-leucine and TAB-proline. Therefore, alpha-aminocaprylic acid has been used as the internal standard instead of noreleucine. TAB-alpha-aminocaprylic acid appears as a separate peak between TAB-serine and TAB-cysteine.
    [Abstract] [Full Text] [Related] [New Search]