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  • Title: Rad53 FHA domain associated with phosphorylated Rad9 in the DNA damage checkpoint.
    Author: Sun Z, Hsiao J, Fay DS, Stern DF.
    Journal: Science; 1998 Jul 10; 281(5374):272-4. PubMed ID: 9657725.
    Abstract:
    The Rad53 protein kinase of Saccharomyces cerevisiae is required for checkpoints that prevent cell division in cells with damaged or incompletely replicated DNA. The Rad9 protein was phosphorylated in response to DNA damage, and phosphorylated Rad9 interacted with the COOH-terminal forkhead homology-associated (FHA) domain of Rad53. Inactivation of this domain abolished DNA damage-dependent Rad53 phosphorylation, G2/M cell cycle phase arrest, and increase of RNR3 transcription but did not affect replication inhibition-dependent Rad53 phosphorylation. Thus, Rad53 integrates DNA damage signals by coupling with phosphorylated Rad9. The hitherto uncharacterized FHA domain appears to be a modular protein-binding domain.
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