These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Transcriptional regulation of the c-H-ras1 gene by the P53 protein is implicated in the development of human endometrial and ovarian tumours.
    Author: Zachos G, Spandidos DA.
    Journal: Oncogene; 1998 Jun 11; 16(23):3013-7. PubMed ID: 9662334.
    Abstract:
    The human c-H-ras1 gene contains within the first intron a p53 element, which functions as a transcriptional enhancer. Using nuclear extracts from human endometrial and ovarian tumours in gel retardation assays, we examined the binding levels of the P53 protein to the H-ras element in tumour versus the adjacent normal tissue. Elevated P53 binding in the tumour tissue was found in 5/12 (42%) endometrial and in 2/5 (40%) ovarian specimens and these cases were found to overexpress wild-type P53. Loss of P53 binding to the H-ras element due to p53 mutations, was observed in 3/12 (25%) endometrial and in 1/5 (20%) ovarian cases. Similar P53 binding levels to the H-ras element were found in 4/12 (33%) endometrial and in 2/5 (40%) ovarian pairs showing normal expression of wild-type P53. Overexpression of the Ras p21 protein correlated with elevated binding and increased nuclear levels of wild-type P53. Our results suggest that P53 protein alterations, participate in the development of human gynecological neoplasias through aberrant transcriptional regulation of the H-ras proto-oncogene.
    [Abstract] [Full Text] [Related] [New Search]