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  • Title: Determination of quercetin in human plasma by HPLC with spectrophotometric or electrochemical detection.
    Author: Jones DJ, Lim CK, Ferry DR, Gescher A.
    Journal: Biomed Chromatogr; 1998; 12(4):232-5. PubMed ID: 9667028.
    Abstract:
    A reversed-phase high-performance liquid chromatographic method for the determination of quercetin in human plasma following intravenous infusion is described. Quercetin in plasma was extracted with methanol-dimethyl sulphoxide (4:1 v/v) and separated on a C18 Hypersil-BDS column with 44% (v/v) methanol in 0.1 M ammonium acetate (pH 5.15) containing 0.27 mM EDTA as the mobile phase. The drug was detected specifically and sensitively at its absorption maximum of 375 nm, or electrochemically, with a detection limit of 80 ng/mL and 2 ng/mL, respectively.
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