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  • Title: Effect of high glucose concentration on proinsulin biosynthesis and conversion by human islets.
    Author: Usac EF, Nadal B, Gasa R, Fernandez-Alvarez J, Gomis R.
    Journal: Biochem Biophys Res Commun; 1998 Jul 09; 248(1):186-9. PubMed ID: 9675108.
    Abstract:
    In the present study we investigate whether glucose concentration could have an effect on proinsulin biosynthesis and processing. We cultured control human islets under chronic high and low glucose concentrations. After the culture period, islets were pulse-labeled and chased for different periods of time. Proteins from islets were collected, insulin immunoprecipitated, and analyzed by alkaline-urea gel electrophoresis. We have found an accelerated rate of proinsulin conversion by those islets exposed to high glucose concentration (at 24.4 mM of glucose), but not by those islets cultured at low glucose concentration (at 5.5 mM of glucose). However, we do not observe any decrease or increase on newly proinsulin synthesis in any of these conditions.
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