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  • Title: Immunoelectron microscopic localisation of the OMP90 family on the outer membrane surface of Chlamydia psittaci.
    Author: Longbottom D, Findlay J, Vretou E, Dunbar SM.
    Journal: FEMS Microbiol Lett; 1998 Jul 01; 164(1):111-7. PubMed ID: 9675857.
    Abstract:
    The putative outer membrane location of the OMP90 (formerly POMP) family from the ovine abortion strain of Chlamydia psittaci was investigated by immunoelectron microscopy. Using a non-embedding technique, antigens were shown to be localised on the outer membrane surface of both elementary and reticulate bodies, the infectious and non-infectious forms of Chlamydiae respectively. Antibodies affinity-purified against the expressed amino- and carboxy-terminal halves of one of the family members. OMP90A, demonstrated that the amino half is surface-exposed while the carboxyl half is most probably localised internally. Surface localisation on elementary bodies indicates the importance of these proteins as protective antigen candidates.
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