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  • Title: Thin-layer chromatographic determination of monensin in feeds: screening method.
    Author: Landgraf WW, Ross PF.
    Journal: J AOAC Int; 1998; 81(4):844-7. PubMed ID: 9680710.
    Abstract:
    Monensin is extracted from feed with methanol and purified by solvent-partitioning solid-phase extraction. After solvent reduction, monensin is separated by thin-layer chromatography on silica gel and visualized by color development with vanillin. No false-positive results were obtained in validation studies by submitting or peer laboratories when blank samples were analyzed. Three of 20 samples spiked with 5 ppm monensin were reported as containing no monensin. All samples spiked with 10 ppm monensin were reported positive for monensin.
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