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  • Title: Precise determination of RNA-protein contact sites in the 50 S ribosomal subunit of Escherichia coli.
    Author: Thiede B, Urlaub H, Neubauer H, Grelle G, Wittmann-Liebold B.
    Journal: Biochem J; 1998 Aug 15; 334 ( Pt 1)(Pt 1):39-42. PubMed ID: 9693099.
    Abstract:
    RNA-protein cross-linked complexes were isolated and purified to obtain precise data about RNA-protein contact sites in the 50 S ribosomal subunit of Escherichia coli. N-terminal microsequencing and matrix-assisted laser desorption ionization MS were used to identify the cross-linking sites at the amino acid and nucleotide levels. In this manner the following contact sites of five ribosomal proteins with the 23 S rRNA were established: Lys-67 of L2 to U-1963, Tyr-35 of L4 to U-615, Lys-97 of L21 to U-546, Lys-49 of L23 to U-139 or C-140 and Lys-71 and Lys-74 of L27 to U-2334.
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