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  • Title: Two-dimensional electrophoresis of proteins in an immobilized pH 4-12 gradient.
    Author: Görg A, Boguth G, Obermaier C, Weiss W.
    Journal: Electrophoresis; 1998 Jun; 19(8-9):1516-9. PubMed ID: 9694305.
    Abstract:
    For checking theoretical two-dimensional (2-D) maps derived from sequenced genomes, indicating that nonnegligible amounts of proteins up to pH 12 are to be expected, a wide-range immobilized pH 4-12 gradient was generated. Depending on the extraction method of sample preparation, proteins with pls up to pH 12 are detected in a single gel. Highly reproducible protein patterns focused to the steady state with round-shaped spots up to pH 12 are obtained with the standard protocol originally described in 1988 (Görg et al., Electrophoresis 1988, 9, 531-546).
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