These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Effect of bile duct obstruction on hepatic uptake of 1-methyl-4-phenylpyridinium in the rat.
    Author: Martel F, Calhau C, Hipólito-Reis C.
    Journal: Pharmacol Res; 1998 Jun; 37(6):497-504. PubMed ID: 9695124.
    Abstract:
    Previous studies have demonstrated that the organic cation 1-methyl-4-phenylpyridinium (MPP+) is avidly taken up by rat freshly isolated hepatocytes through at least two distinct transport mechanisms: the type I hepatic transporter of organic cations and P-glycoprotein. In this study, the effects of extrahepatic cholestasis induced by bile duct ligation for 4 days on the uptake of [3H]MPP+ by rat freshly isolated hepatocytes and liver slices were determined. Bile duct ligation produced no significant alterations in the characteristics of [3H]MPP+ uptake by freshly isolated hepatocytes. The strong correlation found between the effect of various drugs on [3H]MPP+ uptake by hepatocytes from control and treated rats (r = 0.958; P < 0.0001; n = 15) suggests that neither the type I hepatic transporter of organic cations nor P-glycoprotein were affected by bile duct ligation. On the contrary, uptake of [3H]MPP+ by liver slices was markedly changed after bile duct ligation: (1) there was a significant increase (approximately equal to 40%) in the amount of [3H]MPP+ taken up by liver slices from bile duct-ligated rats; (2) there was no correlation between the effect of various drugs on [3H]MPP+ uptake by liver slices from control and treated rats (r = 0.772; P= 0.072; n = 6). On the basis of (1) the lack of effect of bile duct ligation on [3H]MPP+ uptake by isolated hepatocytes; and (2) the profound morphological alterations of liver tissue observed 4 days after bile duct ligation (increase in volume density of bile ductules, ductular cells and infiltration of inflammatory cells), we suggest that non-parenchymal liver cells have an important participation in the hepatic uptake of [3H]MPP+ after bile duct ligation in the rat.
    [Abstract] [Full Text] [Related] [New Search]