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  • Title: [Arguments in favor of the extramitochondrial origin of the acetyl radical of acetylcholine synthetized in rat striatum synaptosomes].
    Author: Lefresne P, Beaujouan JC, Glowinski J.
    Journal: C R Acad Hebd Seances Acad Sci D; 1978 Apr 17; 286(15):1165-7. PubMed ID: 96977.
    Abstract:
    Synaptosomes prepared from Rat striatum were loaded with (2-14C) pyruvate at 0 degree C, in the course of a 1 hr. preincubation. When temperature was raised to 37 degrees C, the intra-synaptosomal (2-14C) pyruvate gave rise to 14CO2 but not to (14C) ACh. These results exclude a mitochondrial origin of the acetyl moiety of ACh.
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