These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Bovine lens leucine aminopeptidase. A study of possible roles for the thiol groups in holo- and apoenzyme.
    Author: Frohne M, Kettmann U.
    Journal: Acta Biol Med Ger; 1976; 35(3-4):353-7. PubMed ID: 970044.
    Abstract:
    The apoenzyme of leucine aminopeptidase has to be prepared either under inert conditions or in the presence of 2-thioethanol. Due to the removal of essential zinc ions, buried -SH groups are exposed. These -SH groups show a higher reactivity than the six -SH groups accessible in the native zinc enzyme. During the incubation of the zinc enzyme with guanidine hydrochloride, up to 36 -SH groups are titratble in dependence on the concentration of guanidine hydrochloride. The -SH groups can be distinguished by their reactivity towards Ellman's reagent and can be differentiated into four classes with respect to their function.
    [Abstract] [Full Text] [Related] [New Search]