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  • Title: Ascidian mitochondrial tRNA(Met) possessing unique structural characteristics.
    Author: Kondow A, Yokobori S, Ueda T, Watanabe K.
    Journal: Nucleosides Nucleotides; 1998; 17(1-3):531-9. PubMed ID: 9708361.
    Abstract:
    Methionine tRNA was purified from muscle mitochondria of the ascidian Halocynthia roretzi and its RNA sequence was determined. Analysis of the nucleotide sequence revealed that unlike most metazoan mitochondrial tRNAs(Met), which have a highly conserved cytidine (C) or C-derivative at the wobble position, the H. roretzi mitochondrial tRNA(Met) possesses 5-carboxymethylaminomethyluridine (cmnm5U) at the first position of the anticodon. This is the first report of a single mitochondrial tRNA(Met) species having uridine (U) or a U-derivative at the wobble position.
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