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  • Title: The microdetermination of copper in plasma protein fractions.
    Author: Delves HT.
    Journal: Clin Chim Acta; 1976 Sep 20; 71(3):495-500. PubMed ID: 971538.
    Abstract:
    Electrophoresis on cellulose acetate membranes and electrothermal atomization for atomic absorption spectrophotometry have been combined to allow the determination of copper in protein fractions separated from only 2 mul volumes of serum. The sensitivity of the method is 5.2 X 10(-11) g of copper for 1% absorption at 324.7 nm, and the precision of replicate analyses at the 1.7-ng level is 0.086 (Relative Standard deviation). The mean recovery of copper from the alpha2-protein fraction of a serum sample was 94%. The method has been applied to a study of the changes in the copper content of the various protein fractions of sera from patients with Menkes' syndrome.
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