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  • Title: Aqueous two-phase affinity partitioning of biotinylated liposomes using neutral avidin as affinity ligand.
    Author: Ekblad L, Kernbichler J, Jergil B.
    Journal: J Chromatogr A; 1998 Jul 31; 815(2):189-95. PubMed ID: 9718699.
    Abstract:
    Biotinylated small unilamellar liposomes were affinity partitioned in an aqueous poly(ethylene glycol)-dextran two-phase system using avidin coupled to dextran as affinity ligand. In the absence of affinity ligand more than 90% of the liposomes partitioned in the poly(ethylene glycol)-rich top phase, whereas in its presence more than 95% partitioned in the dextran-rich bottom phase. For this redistribution to occur 10 mM and above of lithium sulphate, or other appropriate salts, had to be added to the two-phase system. Without added salt the liposomes with complexed avidin-dextran instead partitioned in the top phase. An extended mixing time for the system was required for maximum redistribution. Less than two biotin residues per liposome, coupled via a C6 spacer arm, was required to redistribute the liposomes to the bottom phase.
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