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  • Title: Gene organization and transcriptional analysis of the Spiroplasma citri rpsB/tsf/x operon.
    Author: Le Dantec L, Bové JM, Saillard C.
    Journal: Curr Microbiol; 1998 Oct; 37(4):269-73. PubMed ID: 9732535.
    Abstract:
    The nucleotide sequence of a 6863-bp Spiroplasma citri DNA fragment comprising the spiralin gene was determined. Sequence analysis revealed eight putative ORFs that encode ribosomal protein S2, elongation factor Ts, spiralin, 6-phosphofructokinase, pyruvate kinase, and three unidentified proteins (A, B, and X). The gene organization reported here is different from that previously published. Northern blot analysis of rpsB, tsf, and x transcripts indicates that these genes are organized into a single transcriptional unit (operon). However, the detection of an additional transcript corresponding to the rpsB gene alone suggests that a transcriptional mechanism should occur in the 3' region of the rpsB gene, allowing a conditional transcription termination.
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