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  • Title: Production of trehalose synthase from a basidiomycete, Grifola frondosa, in Escherichia coli.
    Author: Saito K, Yamazaki H, Ohnishi Y, Fujimoto S, Takahashi E, Horinouchi S.
    Journal: Appl Microbiol Biotechnol; 1998 Aug; 50(2):193-8. PubMed ID: 9763690.
    Abstract:
    The genomic DNA and cDNA for a gene encoding a novel trehalose synthase (TSase) catalyzing trehalose synthesis from alpha-D-glucose 1-phosphate and D-glucose were cloned from a basidiomycete, Grifola frondosa. Nucleotide sequencing showed that the 732-amino-acid TSase-encoding region was separated by eight introns. Consistent with the novelty of TSase, there were no homologous proteins registered in the data-bases. Recombinant TSase with a histidine tag at the NH2-terminal end, produced in Escherichia coli, showed enzyme activity similar to that purified from the original G. frondosa strain. Incubation of alpha-D-glucose 1-phosphate and D-glucose in the presence of recombinant TSase generated trehalose, in agreement with the enzymatic property of TSase that the equilibrium lay far in the direction of trehalose synthesis.
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