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  • Title: Mycobacterium avium complex strains from AIDS patients belong to a homogeneous group ascribed by rRNA typing methods.
    Author: Peillon R, Drouet E, Wilmotte R, Cotte L, Panteix G, de Montclos H.
    Journal: Res Microbiol; 1997 Nov; 148(8):683-90. PubMed ID: 9765853.
    Abstract:
    16S rRNA RFLP analysis of Mycobacterium avium complex (MAC) strains isolated from 25 AIDS patients led to identification of seven ribotypes. The same ribotype was determined for strains from 19 patients with and without disseminated disease. When isolates representing the seven ribotypes were examined for their internal transcribed spacer (ITS) between the 16S and 23S rRNA gene nucleotide sequence, four different sequences, including a new ITS type, were recovered. All isolates with the most common ribotype belonged to the sequevar Mav-B. When MAC strains from AIDS patients were compared by ITS sequencing and ribotyping, a significant degree of homogeneity was observed. The discriminatory level reached with ribotyping might be useful for grouping isolates from different clinical sources.
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