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  • Title: Expression and cellular location of endothelin-1 mRNA in rat liver following endotoxemia.
    Author: Liu B, Zhou J, Chen H, Wang D, Hu D, Wen Y, Xiao N.
    Journal: Chin Med J (Engl); 1997 Dec; 110(12):932-5. PubMed ID: 9772405.
    Abstract:
    OBJECTIVE: To study the effect of endotoxin on the alteration of the transcription, expression and cellular location of endothelin-1 (ET-1) mRNA in the hepatic tissue. METHODS: Wistar rats were divided into control and endotoxic group. The rats in the control group were injected with saline, and those in the endotoxic group with endotoxin at a dose of 10 mg.kg-1 body wt. ET-1 hepatic homogenate was assayed by radioimmunoassay at 3, 6, 9, 12 and 24 h after endotoxin administration. Dot blot was used to identify and quantify ET-1 mRNA of the hepatic tissue. Hybridization of ET-1 of the hepatic tissue was proceeded at 3, 6, 12 and 24 h after endotoxin administration. RESULTS: ET-1 concentrations and the level of ET-1 mRNA increased rapidly and reached the peak at 6 h, and remained high at 24 h after endotoxin administration. By in situ hybridization, ET-1 mRNA was found in hepatic sinusoids, endothelial cells of portal vein and kupffer cells. CONCLUSIONS: Endotoxin may be the principal stimulating factor for expression of ET-1 mRNA in hepatic tissue. Endotoxin may affect transcription and translation level of ET-1, leading to increase of the synthesis and release of ET-1. The hepatic vascular endothelial cells, hepatic sinusoids and Kupffer cells all synthesize and release ET-1.
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