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  • Title: Pitfalls in the sensitivity of HBsAg detection.
    Author: Babeş VT, Aderca I.
    Journal: Virologie; 1978; 29(2):87-91. PubMed ID: 97861.
    Abstract:
    A number of 1800 serum samples from apparently healthy subjects were tested by immunodiffusion (ID) and counterimmunoelectrophoresis (CIE) with a view to confirming the presence of HBsAg; results were uncertain in 21% of the cases. The enzyme immunoassay (ELISA kits) used to elucidate these uncertain reactions showed half of them to be negative. About 3% of the remaining sera, HBsAg-positive by ELISA, were positive by ID, but constantly negative by repeated CIE tests. Some possible explanations are suggested and the implications of such pitfalls in the interpretation of sero-epidemiological analyses are discussed.
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