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  • Title: Ouabain-insensitive Na(+)-ATPase activity of Malpighian tubules from Rhodnius prolixus.
    Author: Caruso-Neves C, Meyer-Fernandes JR, Saad-Nehme J, Proverbio F, Marín R, Lopes AG.
    Journal: Comp Biochem Physiol B Biochem Mol Biol; 1998 Apr; 119(4):807-11. PubMed ID: 9787771.
    Abstract:
    In the present paper, we show the existence of a furosemide-sensitive Na(+)-stimulated, Mg(2+)-dependent ATPase activity in cell lysates of Malpighian tubular cells from Rhodnius prolixus, which could be the biochemical expression of the Na(+)-pump. The main characteristics of this activity are: (1) K0.5 for Na+ = 1.49 +/- 0.18 mM, (2) Vmax = 2.8 +/- 0.1 nmol inorganic orthophosphate (Pi).mg prot-1.min-1, (3) it is fully abolished by 2 mM furosemide, (4)it is insensitive to ouabain concentrations up to 10(-2) M, (5) it is sensitive to the presence of vanadate in the incubation medium indicating it to be a P-type ATPase, and (6) it is stimulated by nanomolar concentrations of Ca2+ in the incubation medium.
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