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Title: The isolated H4-H5 cytoplasmic loop of Na,K-ATPase overexpressed in Escherichia coli retains its ability to bind ATP. Author: Obsil T, Merola F, Lewit-Bentley A, Amler E. Journal: Gen Physiol Biophys; 1998 Jun; 17 Suppl 1():52-5. PubMed ID: 9789756. Abstract: The H4-H5 loop of the alpha-subunit of mouse brain Na,K-ATPase was expressed and isolated from E.coli cells. Using fluorescence analogues of ATP, this loop was shown to retain its capability to bind ATP. Isolation of a soluble H4-H5 loop with the native ATP binding site is a critical step for detailed studies of the molecular mechanism of ATP binding and utilisation.[Abstract] [Full Text] [Related] [New Search]