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  • Title: Characterization of Borrelia burgdorferi strains in Lyme arthritis.
    Author: Eiffert H, Karsten A, Thomssen R, Christen HJ.
    Journal: Scand J Infect Dis; 1998; 30(3):265-8. PubMed ID: 9790135.
    Abstract:
    In the study presented, we investigated whether Lyme arthritis is associated with a particular Borrelia burgdorferi genospecies. Using the PCR technique, in 7/11 samples of synovial fluid of patients with Lyme arthritis a part of the ospA-gene was identified and the strains characterized by sequencing of the amplified DNA. Borrelia burgdorferi sensu stricto was found in 3 patients, B. garinii in 3, and B. afzelii in 1 patient. In conclusion, Lyme arthritis is caused by all 3 human pathogenetic genospecies which are actually known. For clinical practice PCR proved to be a rather insensitive diagnostic method, but may confirm the diagnosis of Lyme arthritis in doubtful cases.
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