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  • Title: [Determination of puerarin in gegen ginlian tablets by RP-HPLC].
    Author: Tan S, Qiu X, Li G, Zhao B, Liang Y.
    Journal: Zhongguo Zhong Yao Za Zhi; 1996 Dec; 21(12):732-3, 761-2. PubMed ID: 9812678.
    Abstract:
    A quantitative method was developed for the determination of puerarin in Gegen Qinglian Tablets by reversed phase HPLC. Chromatographic conditions included column ODS-C18, column temperature: 35 C, UV detector: 250 nm, mobile phase: EtOH-H2O (23:77), flow rate: 1 ml/min. The number of theoretical plates calculated for puerarin peak was no less than 2000. The standard curve was linear in the concentration range of 5-80 micrograms/ml, and the correlation coefficient was 0.9999. The average recovery and the relative standard deviation were 97.6% and 1.8% respectivily.
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