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  • Title: Characterization and selectivity of catalytic antibodies from human serum with RNase activity.
    Author: Vlassov A, Florentz C, Helm M, Naumov V, Buneva V, Nevinsky G, Giegé R.
    Journal: Nucleic Acids Res; 1998 Dec 01; 26(23):5243-50. PubMed ID: 9826744.
    Abstract:
    IgG purified from sera of several patients with systemic lupus erythematosus and hepatitis B are shown to present RNA hydrolyzing activities that are different from the weak RNase A-type activities found in the sera of healthy donors. Further investigation brings evidence for two intrinsic activities, one observed in low salt conditions and another specifically stimulated by Mg2+ions and distinguishable from human sera RNases. Cleavage of RNA substrates by the latter activity is not sequence-specific but sensitive to both subtle conformational and/or drastic folding changes, as evidenced by comparative analysis of couples of structurally well-studied RNA substrates. These include yeast tRNAAsp and its in vitro transcript and human mitochondrial tRNALys-derived in vitro transcripts. The discovery of catalytic antibodies with RNase activities is a first step towards creation of a new generation of tools for the investigation of RNA structure.
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