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  • Title: Regulation of intracellular pH in hamster preimplantation embryos by the sodium hydrogen (Na+/H+) antiporter.
    Author: Lane M, Baltz JM, Bavister BD.
    Journal: Biol Reprod; 1998 Dec; 59(6):1483-90. PubMed ID: 9828196.
    Abstract:
    This study was an investigation of the mechanisms for the regulation of intracellular pH (pHi) by hamster preimplantation embryos. The resting pH values of hamster embryos were similar at the 1-cell (7. 19 +/- 0.34), 2-cell (7.21 +/- 0.21), and 8-cell (7.22 +/- 0.41) stages. Cleavage-stage hamster embryos alleviated intracellular acidosis by activity of the Na+/H+ antiporter. The rate of recovery from acidosis was similar for embryos at 1-cell, 2-cell, and 8-cell stages. When Na+/H+ antiporter activity was inhibited by either incubation in Na+-free medium or the presence of an inhibitor, pHi was unable to recover to initial levels. Instead, pHi remained acidic. The Na+/H+ antiporter was also found to contribute to baseline pH regulation, as incubation in Na+-free medium resulted in an immediate intracellular acidification. The set point for Na+/H+ antiporter was pH 7.14. There was no evidence at any developmental stage for activity of either Na+-dependent HCO3-/Cl- exchanger or H+-ATPase in the regulation of pHi. Inhibition of the Na+/H+ antiporter by an amiloride derivative significantly reduced the ability of 2-cell embryos to develop in culture when challenged with acidosis, indicating that the Na+/H+ antiporter is an essential regulator of pHi.
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