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  • Title: Optimization of an antibiotic residue screening test, based on inhibition of Bacillus subtilis BGA, with experimental design.
    Author: Koenen-Dierick K, De Beer JO.
    Journal: Food Addit Contam; 1998 Jul; 15(5):528-34. PubMed ID: 9829036.
    Abstract:
    Experimental design was applied to an agar-diffusion inhibition test for the screening of residues of veterinary antimicrobial drugs in slaughter animals. The effects and interactions of four independent parameters were studied: the dextrose content of the culture medium, the trimethoprim concentration in the culture medium, the thickness of the agar-layer and the preincubation time. The effects on the inhibition zones of sulphadimidine, oxytetracycline, streptomycin and tylosin, substances of four different antibiotic groups were measured. An optimized multi-residue test was established with the parameter values that yielded the best sensitivity for the four substances.
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