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  • Title: A novel allosterically trans-activated ribozyme, the maxizyme, with exceptional specificity in vitro and in vivo.
    Author: Kuwabara T, Warashina M, Tanabe T, Tani K, Asano S, Taira K.
    Journal: Mol Cell; 1998 Nov; 2(5):617-27. PubMed ID: 9844634.
    Abstract:
    We have constructed an allosterically controllable novel enzyme (designated maxizyme) that can be transcribed in vivo under the control of a human tRNA(Val) promoter. The maxizyme has sensor arms that can recognize target sequences, and in the presence of such a target sequence only, it can form a cavity that can capture catalytically indispensable Mg2+ ions. As a target for a demonstration of the potential utility of the maxizyme, we chose BCR-ABL mRNA, the translated products of which cause chronic myelogenous leukemia. Only the maxizyme (but not conventional ribozymes) had extremely high specificity and high-level activity, not only in vitro but also in cultured cells including BV173 cells derived from a patient with a Philadelphia chromosome. The maxizyme induced apoptosis only in leukemic cells with this chromosome.
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