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  • Title: A minor groove RNA triple helix within the catalytic core of a group I intron.
    Author: Szewczak AA, Ortoleva-Donnelly L, Ryder SP, Moncoeur E, Strobel SA.
    Journal: Nat Struct Biol; 1998 Dec; 5(12):1037-42. PubMed ID: 9846872.
    Abstract:
    Close packing of several double helical and single stranded RNA elements is required for the Tetrahymena group I ribozyme to achieve catalysis. The chemical basis of these packing interactions is largely unknown. Using nucleotide analog interference suppression (NAIS), we demonstrate that the P1 substrate helix and J8/7 single stranded segment form an extended minor groove triple helix within the catalytic core of the ribozyme. Because each triple in the complex is mediated by at least one 2'-OH group, this substrate recognition triplex is unique to RNA and is fundamentally different from major groove homopurine-homopyrimidine triplexes. We have incorporated these biochemical data into a structural model of the ribozyme core that explains how the J8/7 strand organizes several helices within this complex RNA tertiary structure.
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