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  • Title: [Chromatography of thrombin and antithrombin III on biospecific silochromes].
    Author: Lohins'ka ZV, Oryshchenko NV, Vus MM, Maherovskyĭ IuV, Haĭda HZ, Haĭda AV.
    Journal: Ukr Biokhim Zh (1978); 1998; 70(3):63-8. PubMed ID: 9848182.
    Abstract:
    It was shown that the seaweed sulfopolysaccharides Furcellaria and Phyllophora--furcellaran and phyllophoran--are the competitive inhibitors of thrombin-induced fibrinogen clotting. Biospecific adsorbents for thrombin and antithrombin III (AT III) isolation were synthesized with heparin, phyllophoran and furcellaran as ligands on aminopropylsilochrome and this proteins sorption characteristics on this sorbents in comparisons with heparin-cellulose and heparin-sepharose were investigated. Possible application of the synthesized adsorbents both for thrombin, AT III purification from blood products and for alpha-thrombin, beta and gamma-thrombin separation are discussed.
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