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Pubmed for Handhelds

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  • Title: Obtaining and characterization of EF-hand mutants of recoverin.
    Author: Alekseev AM, Shulga-Morskoy SV, Zinchenko DV, Shulga-Morskaya SA, Suchkov DV, Vaganova SA, Senin II, Zargarov AA, Lipkin VM, Akhtar M, Philippov PP.
    Journal: FEBS Lett; 1998 Nov 27; 440(1-2):116-8. PubMed ID: 9862438.
    Abstract:
    Several EF-hand recoverin mutants were obtained and their abilities to bind to photoreceptor membranes and to inhibit rhodopsin kinase were determined. The mutants with the 'spoiled' 2nd, 3rd or (2nd+3rd) EF-hand structures did not act upon the kinase activity in the microM range of Ca2+ concentrations. Mutations of the 4th EF hand, which 'repaired' its Ca2+-binding activity, resulted in recoverin with three 'working' Ca2+-binding sites. The latter mutant inhibited rhodopsin kinase even more effectively than the wild-type recoverin, containing two working Ca2+-binding structures.
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