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  • Title: Modification of an ion-paired high pressure liquid chromatography for ticarcillin in human serum.
    Author: Zhu Z, Xuan DW, Nightingale CH.
    Journal: Zhongguo Yao Li Xue Bao; 1996 Sep; 17(5):395-8. PubMed ID: 9863157.
    Abstract:
    AIM: To develop a new HPLC assay for the clinical pharmacokinetic study of ticarcillin in human. METHODS: Reverse phase ion-paired HPLC was developed with a Novapak C18 column. The mobile phase consisted of 20% of acetonitrile and 80% of phosphate buffer 10 mmol.L-1 (pH 2.0-2.5) containing tetrapentylammonium bromide of 1 mmol.L-1. The analysis was monitored at 225 nm and the flow rate of the mobile phase was 0.8 mL.min-1. Cefoxitin was used as an internal standard. The sample volume was 200 microL of serum. RESULTS: The assay was linear in the range of 2-80 mg.L-1. The intrarun coefficients of variation (CV) for a ticarcillin 5 mg.L-1 check sample was 4.2% (n = 10), and 2.7% for a 60 mg.L-1 check sample (n = 10), respectively. The inter-run CV (n = 6) were 1.6% (5 mg.L-1) and 2.3% (60 mg.L-1), respectively. The average recovery of the assay was 96.6%. CONCLUSION: This assay was sensitive, precise, and accurate for evaluating the clinical pharmacokinetics of ticarcillin.
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