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  • Title: Globin mRNA contains a sequence complementary to double-stranded region of nuclear pre-mRNA.
    Author: Ryskov AP, Tokarskaya OV, Georgiev GP, Coutelle C, Thiele B.
    Journal: Nucleic Acids Res; 1976 Jun; 3(6):1487-98. PubMed ID: 986644.
    Abstract:
    Melted ds RNA isolated from rabbit bone marrow pre-mRNA was hybridized with excess of globin mRNA which was prepared from rabbit reticulocytes. 7-9% of ds sequences became RNAase-stable and about 30% of the sequences could be bound to poly(U)-Sepharose through poly (A) of mRNA. The size of RNAase-stable hybrid is about 30 nucleotides, that is one fourth of the length of one strand of the ds RNA.
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