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  • Title: Column liquid chromatography determination of vitamins A and E in powdered milk and local flour: a validation procedure.
    Author: Ake M, Fabre H, Malan AK, Mandrou B.
    Journal: J Chromatogr A; 1998 Nov 27; 826(2):183-9. PubMed ID: 9871338.
    Abstract:
    A high-performance liquid chromatography method was developed for the simultaneous routine determination of vitamins A and E in powdered milk and flour made from local plants and purchased from open markets in the Ivory Coast. The method involves saponification followed by extraction with a mixture of organic solvents. The vitamins were resolved by reversed-phase HPLC and detection at a single wavelength. The main tests of method validation were applied to the procedure. The results show the reliability of the analytical method for the intended application.
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