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  • Title: Gram type-specific broad-range PCR amplification for rapid detection of 62 pathogenic bacteria.
    Author: Klausegger A, Hell M, Berger A, Zinober K, Baier S, Jones N, Sperl W, Kofler B.
    Journal: J Clin Microbiol; 1999 Feb; 37(2):464-6. PubMed ID: 9889245.
    Abstract:
    Broad-range PCR has proven to be useful for the detection of bacteria. A set of broad-range PCR primers directed against conserved regions in the 16S rRNA gene was designed to specifically amplify either gram-positive or gram-negative bacteria. The gram type-specific broad-range PCR correctly classified all 62 pathogenic species tested.
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