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  • Title: The detection of penicillin in buttermilk.
    Author: Pekkanen TJ, Raevuori M.
    Journal: Nord Vet Med; 1976; 28(7-8):381-4. PubMed ID: 989169.
    Abstract:
    When 59 buttermilk samples were tested for the presence of antibiotics or chemostherapeutic substances by a method using the spores of Bacillus stearothermophilus var. chalidolactis all samples gave a positive reaction corresponding to about 0.01 IU penicillin per ml. When the buttermilk samples were diluted 1:1 (v/v) by a phosphate buffer, pH 6.8, only 2 were positive which were confirmed to contain penicillin. The mean pH of the unbuffered buttermilks was found to be 4.53 +/- 0.11 and when buffered 6.46 +/- 0.05. An in vitro addition of benzylpenicillin to buttermilk, to a concentration of 0.01 IU per ml, resulted in a significantly higher concentration of penicillin in the casein fraction than in the buttermilk or in the whey (Table I).
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