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  • Title: Analysis of suckling-induced changes in adenohypophyseal prolactin concentration in the lactating rat by three assay methods.
    Author: Nicoll CS, Mena F, Nichols CW, Green SH, Tai M, Russel SM.
    Journal: Acta Endocrinol (Copenh); 1976 Nov; 83(3):512-21. PubMed ID: 989994.
    Abstract:
    Suckling-induced changes in adenohypophyseal prolactin (PRL) concentrations in lactating rats were measured in two experiments by three assays: a disc electrophoretic assay (DEA), a bioassay (BA) and a radioimmunoassay (RIA). Substantial discrepancies among the three assays were found in the absolute amounts of pituitary PRL measured and in the changes in adenohypophyseal PRL concentration that were recorded between suckling intervals. The results indicate that in dynamic states of secretory activity the correspondence among BA, RIA and DEA estimates of pituitary PRL concentration is poor.
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