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  • Title: High-performance liquid chromatography-coulometric electrochemical detection of ubiquinol 10, ubiquinone 10, carotenoids, and tocopherols in neonatal plasma.
    Author: Finckh B, Kontush A, Commentz J, Hübner C, Burdelski M, Kohlschütter A.
    Journal: Methods Enzymol; 1999; 299():341-8. PubMed ID: 9916213.
    Abstract:
    A micromethod for the rapid simultaneous determination of several lipophilic antioxidants in plasma from newborn infants is presented. Because only 5 microliters of plasma is required, the procedure lends itself for repetitive use in very immature infants at risk for developing so-called "oxygen radical diseases of the premature." The method allows continuous monitoring of antioxidants in such patients and can easily be combined with monitoring other parameters of interest in this context. Reuse of blood samples taken routinely for the determination of hematocrit and bilirubin concentration is possible, reducing the blood volume required to be taken for the oxygen radical-related studies to virtually zero.
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