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  • Title: Ferrocene-conjugated m-phenylenediamine conducting polymer-incorporated peroxidase biosensors.
    Author: Mulchandani A, Pan S.
    Journal: Anal Biochem; 1999 Feb 01; 267(1):141-7. PubMed ID: 9918666.
    Abstract:
    The development and characteristics of a reagentless amperometric biosensor employing horseradish peroxidase incorporated in an electrochemically deposited ferrocene-modified phenylenediamine film on a glassy carbon electrode is reported. The horseradish peroxidase/poly(m-aminoanilinomethylferrocene)- modified glassy carbon electrode reagentless biosensor measured hydrogen peroxide and other organic peroxides in both aqueous and organic medium by reduction at a low applied potential of -0.05 V (vs Ag/AgCl) without interference from molecular oxygen. When modified with glucose oxidase, the new bienzyme electrode measured glucose sensitively and selectively, demonstrating the suitability of the above peroxide biosensor for other oxidoreductase enzyme-based biosensors.
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