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  • Title: Two-dimensional crystals of reconstituted beta-subunits of the chaperonin TF55 from Sulfolobus shibatae.
    Author: Koeck PJ, Kagawa HK, Ellis MJ, Hebert H, Trent JD.
    Journal: Biochim Biophys Acta; 1998 Dec 08; 1429(1):40-4. PubMed ID: 9920382.
    Abstract:
    We have obtained 2-dimensional crystals of the beta-subunits of the chaperonin TF55 from Sulfolobus shibatae reconstituted into oligomers in the absence of alpha-subunits. The subunits form rings with 9-fold rotational symmetry which arrange themselves in a trigonal lattice. From electron micrographs of negatively stained specimens we have calculated a projection map in plane group p312 showing the rings in top-view.
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