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  • Title: Cross-linked telomere-protein complexes from chicken erythrocyte nuclei: isolation by a new procedure.
    Author: Cervoni L, Ferraro A, Eufemi M, Altieri F, Chichiarelli S, Turano C.
    Journal: Biochem Biophys Res Commun; 1999 Jan 27; 254(3):517-21. PubMed ID: 9920771.
    Abstract:
    DNA-protein cross-linkages were produced in intact nuclei of chicken erythrocytes by the action of cis-diammine dichloroplatinum. The telomeric DNA-protein cross-linked complexes were then isolated by hybridization with a biotinylated oligonucleotide and selective binding on immobilized streptavidin. Two main nonhistone proteins were present in the purified complexes, migrating in SDS-gel electrophoresis with apparent molecular masses of 66 and 58 kDa, respectively. Although the identity of these two proteins is still unknown, it is significant that two proteins with similar electrophoretic behavior have been described as constituents of the human telomeric complexes. This procedure could also be applied to the isolation of DNA-protein cross-linked complexes containing any chosen DNA sequence.
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