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  • Title: Expression of cytokine messenger ribonucleic acids in the bovine corpus luteum.
    Author: Petroff MG, Petroff BK, Pate JL.
    Journal: Endocrinology; 1999 Feb; 140(2):1018-21. PubMed ID: 9927338.
    Abstract:
    There is considerable evidence that luteolysis in the cow and other species involves components of the immune system. In this study, we examined the expression of the mRNAs for TNF-alpha, IFN-gamma, IL-1beta, IL-2, and IL-2 receptor (IL-2R) by reverse transcription-polymerase chain reaction (RT-PCR) using bovine-specific primers. Expression was examined in corpora lutea (CL) of the early (day 5), mid (days 11-12), and late (day 18) luteal phase, and at 1, 4, and 24 hours following a luteolytic dose of prostaglandin (PG) F2alpha. Tumor necrosis factor-alpha, IFN-gamma, and IL-1beta mRNAs were detectable by RT-PCR at all stages of the cycle examined. Densitometric intensities of the electrophoresed IFN-gamma PCR products revealed a drop in RNA expression during late diestrus and at one hour of prostaglandin-induced luteolysis (P < 0.05). The mRNA for TNF-alpha seemed to remain constant during the cycle, and rose slightly during luteolysis. Interleukin-1beta mRNA also did not vary during the cycle or during luteolysis. Finally, expression of mRNAs for IL-2 and IL-2 receptor was not evident in CL by the methods employed in this study. These results are the first to describe mRNA expression for TNF-alpha, IFN-gamma, and IL-1beta in the bovine corpus luteum, and support a role for these cytokines in luteal function and regression.
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