These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: The aminoacceptor stem of the yeast tRNA(Lys) contains determinants of mitochondrial import selectivity.
    Author: Kazakova HA, Entelis NS, Martin RP, Tarassov IA.
    Journal: FEBS Lett; 1999 Jan 15; 442(2-3):193-7. PubMed ID: 9929000.
    Abstract:
    Yeast tRNA(Lys)CUU is nucleus-encoded and is partially imported into the mitochondria. Another lysine isoacceptor, tRNA(Lys)SUU, is also nucleus-encoded but is not imported. These two tRNAs differ in 21 bases. We have previously localised import selectivity determinants in the anticodon arm. By in vitro import of mutant transcripts and by expression of mutant tRNA genes in vivo we show here that the first base pair (1:72) and the discriminator base 73 are also relevant to import selectivity. Replacement of bases 1:72 in tRNA(Lys)SUU by those of tRNA(Lys)CUU makes it importable with a transport efficiency similar to natural.
    [Abstract] [Full Text] [Related] [New Search]