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  • Title: Lymphocyte isolation, rosette formation, and mitogen stimulation in Rhesus monkeys.
    Author: Taylor DW, Marchette NJ, Siddiqui WA.
    Journal: Dev Comp Immunol; 1978 Jul; 2(3):539-46. PubMed ID: 99337.
    Abstract:
    Over 70% of rhesus monkey peripheral mononuclear cells were isolated on sodium metrizoate-ficoll gradients with greater than 98% purity. Rhesus blood contained 47.8% active E, 58.2% total E, and 30.2% EAC rosette forming cells. Optimal conditions for mitogen studies were determined using phytohemagglutinin, concanavalin A, pokeweed mitogen, lipopolysaccharide and streptolysin O.
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